CB1 receptor agonist protects the retina from light induced retinal degeneration

CALTANA L; LOPEZ JJ. ; EM. LÓPEZ; BRUSCO A; M. SOLIÑO

Mar del Plata

Congreso; Juan José López; 2017

SAN2017CB1 RECEPTOR AGONIST PROTECTS THE RETINA FROM LIGHT INDUCED RETINAL DEGENERATION.Soliño M, López EM, Caltana L, Brusco A, and López-Costa JJ.IBCN ¨Prof. E. De Robertis¨, Fac Med, UBA-CONICET; Fac Med, UBA-CONICET. Endocannabinoids are neuromodulators whose effects are mediated by G protein coupled receptors named CB1 and CB2. CB1 agonists play a neuroprotective role in glaucoma models of retinal injury. Light induced retinal degeneration (LIRD) is a model that resembles other human retinal degenerative diseases as AMD. The aim of this work was to evaluate the potential neuroprotective effect of the modulation of CB1 receptor in LIRD. The right eyes of Sprague Dawley rats were intravitreally injected either with ACEA (CB1 agonist), or AM251 (CB1 antagonist) while the left eyes received vehicle as controls. Later, rats were subjected to continuous illumination (12.000 lux) for 24 hs. Retinas were dissected out and processed by Western Blot (WB) using either antibodies to GFAP or to activated Caspase 3. The optical density data were statistically analysed using Student´s t-test and differences were considered significant when p