neuronal cultures exhibit stress related changes when are exposed to preDBT sera

BADOLATI, ADELINA; PUGLIESE, CECILIA; BAEZ M. VERÓNICA; TOMAS DE LIORO, ANNA

Buenos Aires

Simposio; AAIC-Satellite-Symposium; 2018

Alzheimer's Association

Diabetes type 2 (DBT2) is a metabolic disorder clinically characterized by an increase in blood glucose levels. It is well known that DBT2 causes neurodegeneration, however, molecular mechanisms involved in this process are not clear yet. Several hypothesis were proposed in order to elucidate molecular changes and cascades involved in neurodegeneration caused by DBT2. Between them, the ones related to RAGE products, stress signaling and chronic inflammation are the best investigated. On the other hand, pre-diabetes 2 (preDBT) is considered a step before DBT2. PreDBT is thought to be revesible. However it is not well understood if preDBT syndrome per se, or the degeneration to DBT2 syndrome could cause neurodegeneration. In order to better elucidate which mechanisms are involved in synaptic plasticity impairment and neurodegeneration, and if these processes start during preDBT stage, we exposed primary neuronal cultures to human serum extracted form control or preDBT patients and analyzed them by Immunofluorescence against stress, neuronal and glia markers. We found an increase in stress granules when neuronal cultures are incubated for 1 hour with preDBT sera but not with control patients sera. In the other hand, when cultures were incubated with human serum for several days, we observed changes in neuronal morphology as well in glia number only in cultures treated with preDBT sera. These preliminary results would led us to hypothesized that preDBT could cause changes in neurons that are compatible with neurodegeneration