IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Deep hypothermic shock reverses the damage caused by perinatal asphyxia in the rat's striatum
Autor/es:
MANUEL SOLIÑO; ANDRES ACUÑA; CÉSAR FABIÁN LOIDL ; PABLO VAZQUEZ; JUAN JOSE LOPEZ
Reunión:
Congreso; XXXII CONGRESO ANUAL SAN 2017; 2017
Resumen:
The striatum is particularly vulnerable to perinatal asphyxia (PA). The main cells of thisstructure are the median spiny neurons, which are GABAergic calbindin (CB) positiveneurons. At the time of delivery GABA has excitatory properties and the excitotoxicity processcould be mediated through GABA-GABA synapses. The GFAP is an astrocyte protein that isoverexpress after brain injury. The present work aims to quantify CB and GFAP afterexposure to PA in the striatum and to evaluate the therapeutic effect of a short and deephypothermic shock after asphyxia. The uterus was removed by caesarean section and thefetuses were exposed to hypoxia (19 min at 37 C˚) by immersion in water and, also, exposedto a temperature of 10 C˚for 30 min in case of the hypothermic group. Four experimentalgroups of 3-4 rats each were formed. The labeling of CB, GFAP, neuN, DAPI was measured inadult rats. In the PA group there was a significant decrease in CB positive neurons and anincrease in GFAP expression compared to control. Treatment with post-asphyxia hypothermiaprevented changes in CB and GFAP showing expression levels similar to control. Thequantification of NeuN, DAPI did not show differences between groups. Perinatal asphyxiagenerates a decrease in the GABAergic cells of the striatum and increase a marker of braininsult as GFAP. Hypothermia seems to reverse this damage. Deep hypothermia could be asuperlative option to reduce severe disability generated by the PA.