IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ARE CB1R AND CB2R INTERACTING IN THE REGULATION OF ANXIETY?
Autor/es:
NOVAK, LUCIANA; CALTANA, LAURA ROMINA; SORIANO, DELIA; BRUSCO, ALICIA; MADARNAS, CATALINA
Reunión:
Congreso; XXXII CONGRESO ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION EN NEUROCIENCIAS; 2017
Resumen:
The neuromodulatory role of the endocannabinoid system contributes on maintaining the homeostasis of physiological states including mood and emotion. Changes in the expression of the CB1 receptor (CB1R) gene have been related to depressive effects. Mice lacking CB1R exhibited a behavioral state analogous to depression in experimental animals.Previous results, showed that CB1R knock-out mice (CB1-/-) presents an increase in CB2 receptor (CB2R) expression and altered neuronal plasticity.The aim of the study is to evaluate the effect of CB2R agonist (JWH015) and antagonist (AM630) in behavioral paradigms of anxiety and depression in CB1 receptor wild-type mice (CB1+/+) and CB1-/-.CB1+/+ and CB1-/- mice were divided into four groups and were injected with Vehicle, JWH015, AM630 or JWH015+AM630. Open field test (OF) and forced swimming test (FST) were performed 30 minutes and 2hours after injection, respectively.In OF test, time spent in periphery was decreased by JWH015 and increased by AM630 in CB1+/+ meanwhile time spent in central zone was increased by JWH015 and decreased by AM630 in CB1+/+. No differences were observed in CB1-/-.In FST, time of immobilization was increased in CB1-/- respect to CB1+/+. Treatment with agonists or antagonist did not show any differences in CB1-/- or CB1+/+.Since CB2 agonist, JWH015 has an anxiolytic effect in CB1+/+ but has no effect on CB1-/-, we proposed that CB1R and CB2R could be interacting in the regulation of anxiety.