IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Reactive astrogliosis propagation in a model of in vitro scratch injury
Autor/es:
VERONICA MURTA; JERONIMO LUKIN; GERARDO ROSCISZEWSKI; ALBERTO JAVIER RAMOS; BELEN CIERI; VANESA CADENA; JERONIMO AUZMENDI
Lugar:
Buenos Aires
Reunión:
Congreso; 2do Congreso de la Federación de Asociaciones Latinoamericanas y del Caribe de Neurociencias (FALAN); 2016
Institución organizadora:
Federación de Asociaciones Latinoamericanas y del Caribe de Neurociencias (FALAN)
Resumen:
Reactive gliosis propagation is a serious consequence of brain injury and it is still unknown how it can reach very distant regions in the CNS after a focal brain injury. In this work we explored in vitro in purified micro-astroglial mixed culture from rat brain several mechanisms that are proposed to be involved in the propagation of reactive gliosis after a focal injury. Using time lapse experiments we show that a 30 min preincubation with 25 ng/ml LPS (PAMP) or 500 ng/ml HMGB1 (DAMP) facilitated the astroglial migration to the injured area, and migration was effectively blocked by 10 µM of NFkB inhibitor BAY117082 or by chelating extracellular calcium with 20 µM EGTA. Then, we followed the profile of reactive gliosis by analyzing GFAP expression at fixed distances from scratch. We observed a GFAP expression gradient that was increased by 10-20% when we preincubated with LPS or HMGB1 and again was blocked by BAY. The absence of microglia abolished the GFAP gradient except in HMGB-1 treated culture. We conclude that HMGB1 or LPS modifies the profile of reactive gliosis propagation in vitro when microglia is present. We suggest that the simple diffusion of molecules is an insufficient mechanism for explain the gliosis propagation, and probably requires cooperation of microglia.