IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Setting up the experimental conditions to uncover a powerful triumvirate: neurogenesis, serotonin and BDNF
Autor/es:
FOLTRAN, RB; TARAVINI, IRE; LOPEZ STEINMETZ, LC; DIAZ SL; HÖCHT, C
Lugar:
Buenos Aires
Reunión:
Congreso; 2nd FALAN Congress; 2016
Institución organizadora:
FALAN-SAN
Resumen:
Adult hippocampal neurogenesis can be enhanced by factors like chronic administration of antidepressants, in particular, serotonin (5-HT)-selective reuptake inhibitors (SSRIs). Besides increasing serotonergic neurotransmission, SSRIs also increase the hippocampal expression of brain-derived neurotrophic factor (BDNF). All in all, BDNF and 5-HT appear to modulate hippocampal neurogenesis. We aim to dissect the role of these molecules in the neurogenic multistep process. In order to avoid intraperitoneal (i.p.) injections, experimental conditions for SSRIs oral (p.o.) administration were set up. C57Bl/6J female 6-week old mice were treated during 4 weeks as follow: 1) vehicle, 2) fluoxetine (Flx) i.p., 3) Flx p.o., 4) paroxetine (Prx) p.o. Both Flx and Prx were stable in water up to 96 h as measured by HPLC. Serum Flx and Prx levels are under analysis. Mice treated with Flx i.p. shown significantly lower immobility time in the forced swimming test, but no effect was observed in mice receiving p.o. SSRIs. In the novelty suppressed feeding test, all SSRIs treated groups showed a tendency to a decrease in the latency to feed. Neurogenic parameters in the hippocampus will be studied by immunohistochemistry and determined by unbiased stereologically counting. These results will prove useful for a future proposal of a replacement in the administration of SSRIs, improving animal welfare as well as the feasibility of the experimental protocol.