IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CB1R deficiency alters neuronal morphology and synaptic plasticity in the adult mouse hippocampus
Autor/es:
BRUSCO, ALICIA; SORIANO, DELIA; CALTANA, LAURA; CONDE, FLORENCIA
Lugar:
CABA
Reunión:
Congreso; 2nd Congress of the Federation of Latin-American and Caribbean Societies for Neuroscience (FALAN); 2016
Institución organizadora:
SAN
Resumen:
The cannabinoid receptor type 1 (CB1R) is mainly located on presynaptic neurons and modulates synaptic activity. It is widely distributed in the central nervous system and is involved in physiological processes such as memory, learning, motor coordination, anxiety and mood. Genetic deletion of CB1R causes alterations similar to those described in animal models of depression. The aim of this work was to evaluate the neuronalcytoskeleton and synaptic plasticity in the adult hippocampus in CB1R knockout mice (CB1R-/-). This studywas performed using immunofluorescence, Golgi, Western Blot and Electron Microscopy techniques, focusing on the CA1 area. CB1R-/- mice showed a decrease in the expression of the neuronal cytoskeleton components (Nf-160kDa, Nf-200kDa and MAP-2). We also observed a reduction in the number ofprimary projections. In addition, results showed a decrease in Synaptophysin expression, and a tendency to increase the number of vesicles in the synaptic terminal in CB1R-/- mice, probable evidencing changes in neurotransmitter release. The number of synapses was not affected but thepostsynaptic density thickness was reduced. The absence of CB1R alters neuronal architecture in theadult brain, affecting synaptic plasticity and modeling.Such changes might correlate with reported changes in the behavior in CB1R-/- mice.