Regeneration of chicken embryo retina throughout activation of Shh pathway in endogen stem cells.

DI NAPOLI, JENNIFER; NIETO, DENIS ALEJANDRO; DEL RIO-TSONIS, KATIA; FIORE, LUCIANO; SANCHEZ, VIVIANA; OLMOS CARREÑO, CINDY; TERUEL, LUISA R; SCICOLONE, GABRIEL

Buenos Aires

Congreso; 2nd FALAN (Federation of Latinoamerican and Caribean Neuroscience Society ) Congress 2016; 2016

Federation of Latinoamerican and Caribean Neuroscience Society

Regeneration of chicken embryo retina throughout activation of Shh pathway in endogen stem cells.Di Napoli J1 ; Olmos Carreño C1; Fiore L1; Nieto D.A.1; Teruel LR1; Sanchez V1, Del Rio-Tsonis K2, Scicolone G1. 1 IBCN, 2 Miami University.1 Inst de Biol Celular y Neurociencias ?Prof E De Robertis? (UBA-CONICET), Facultad Medicina, UBA.2 Dept. of Biol. , Miami University, Oxford; OH, USA.Retina regeneration in chicken embryos can occur through two pathways: by retinal pigmented epithelium (RPE) transdifferentiation or by the activation of stem cells located in the ciliary margin (CM). In chicks, retina regeneration only proceeds during the fourth day of development (E4) by stimulating with growth factors. Fibroblast growth factor 2 (FGF2) stimulates transdifferentiation and activation of stem cells, whereas sonic hedgehog (Shh) induces activation of stem cells. Transdifferentiation produces an inverted retina without RPE, whereas regeneration from the CM produces a normally oriented and laminated retina. This last form of regeneration is the goal of any regenerative strategy.The aim of this work was to test which treatment presents the highest efficacy to produce a complete retina along all its tangential extension. The Shh pathway and its analog activator SAG were evaluated for this purpose.After retinal removal at E4, we tested different treatments to obtain retinal regeneration: Shh or SAG alone or in combination with FGF2.Activation of the canonical Shh pathway with SAG produces the highest efficacy to obtain a complete retinal regeneration through the activation of stem cells (100% of embryos). We showed that the retinal ganglion cells from this regenerated retina have the same positional information that the retinas during development.These results increase the possibility to obtain an efferent system which establishes topographic ordered connections.CONICET and UBA.