Deep hypothermic shock reverses the loss of calbindin-positive neurons caused by perinatal asphyxia in the rat.

PABLO VÁZQUEZ; ELENA PEÑA; YANINA ROJO; FABIÁN LOIDL.

Buenos Aires

Congreso; 2nd FALAN congress; 2016

SAN

The consequences of perinatal asphyxia (PA) include disorders which may manifest as schizophrenia. Therapeutic hypothermia has shown to reverse some of the symptoms generated by the PA. The purpose of this work is to assess if a short and deep hypothermic shock treatment (HYPPA) can reverse the hypoxic damage on subpopulations of GABAergic cells and reverses the deterioration in social interaction produced by PA. A brief exposure to a deep hypothermic shock reverses the deterioration produced by PA in play soliciting. The PA produced a reduction in the density of calbindin neurons in layer II of the AIC, while HYPPA reversed this effect. Paradoxically, in AIC there was a significant increase in the number of reelin secreting neurons in layers II and III generated by PA and this increase was reversed by. This suggests a compensatory mechanism were reelin neurons trend to compensate the loss of calbindin neurons, at least within AIC. Finally, deep hypothermic shock may be considered as a valuable therapeutic alternative for PA.resumen ibro :Obstetrical complications, particularly in the case of perinatal asphyxia, can often induce lesions that, in the long-term, manifest as schizophrenia. In this study we analyzed the effect of perinatal asphyxia on social behavior, anxiety and locomotive activity in rats using two behavioral paradigms: the open field and the social interaction task. We analyzed the behavior of rats delivered by cesarean section and exposed to 19 min of asphyxia and compared the results with rats born by vaginal delivery and cesarean section. We also subjected a group of animals to asphyxia at low temperature to analyze the neuroprotective effects of hypothermia.The rats exposed to perinatal asphyxia showed deterioration in social interactions that manifested in a decrease in the total number of interactions. The quality of interaction was also diminished, with lower rates of play soliciting. We did not find significant changes in locomotive activity or in anxiety indicators in the open field task in this model, which involves severe/moderated asphyxia. The only exception is an increase in fecal depositions in the group born by c-section. When these parameter are analyzed in the social interaction task the result are the opposite, the asphyctic group shows an increase compared with the cesarean group. Other additional behavioral changes were caused by the c-section itself in the social interaction tasks including a preference for non rough play. With the exception of the c-section rats, we observed a strong correlation between certain types of play, probably generated by an interrelationship between boxing and pinning. The group exposed to asphyxia under hypothermia trend to obtain higher scores than the asphyctic group in most of the parameters analyzed, yet the differences were not significant. We attribute this to the fact that social interaction is very vulnerable to neuronal damage.