IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Consequences of maternal alcoholism on the radial glia.
Autor/es:
ARONNE MP, FONTANET P, GUADAGNOLI T, EVRARD S, BRUSCO A
Lugar:
Buzios, Brasil
Reunión:
Congreso; 1er Congreso IBRO/LARC de Neurociencias de América Latina, Caribe y Península Ibérica (NeuroLatAm); 2008
Institución organizadora:
SAN
Resumen:
Prenatal ethanol exposure (PEE) induces structural disorders in the developing central nervous system (CNS). The relationship between radial glia cells (RGC) and migrating neuroblasts is crucial for the establishment of normally laminated structures. This study was conducted to determine RGC temporal vulnerability as a function of exposure to EtOH during brain development. Pregnant Wistar rats, 200-250 g, were fed one month before and during pregnancy and lactation a liquid diet with 5.9% (w/w) EtOH; their fetuses were compared with that of mothers fed the control liquid diet. Fetal brains were morphologically studied at embryonic days E12, E14, E16 and E18. Vimentin (VIM) cortical expression (an RGC cytoskeletal marker) and S-100b protein (a neurotrophic factor, cytosolic marker of RGC during embryonic development) were decreased in all gestational ages (for VIM control vs EtOH; E12: 27.99 ±0.90 vs 11.85 ±1.27 [P<0.0001]; E14: 23.9 ±0.67 vs 13.44 ±0.55 [P<0.0001]; E16: 19.04 ±0.83 vs 10.07 ±1.05 [P<0.0001]; E18: 16.59 ±0.88 vs 6.71 ±0.33 [P<0.0001]; for S100b control vs EtOH; E12: 19.55 ±1.25 vs 10.56 ±1.41 [P<0.0001]; E14: 21.23 ±0.52 vs 14.55 ±0.41 [P<0.0001]; E16: 15.47 ±0.87 vs 8.28 ±0.85 [P=0.0002]; E18: 13.69 ±1.02 vs 6.71 ±1.23 [P=0.0007]). At birth, treated offspring showed both body weight and cerebral cortex thickness reduction (control vs EtOH; E12: 0.40 ±0.02 vs 0.37 ±0.01 [P=0.1787]; E14: 0.59 ±0.02 vs 0.57 ±0.02 [P=0.548]; E16: 1.55 ±0.07 vs 1 ±0.07 [P<0.0001]; E18: 1.97 ±0.09 vs 1.59 ±0.06 [P=0.0021]). Our data suggest that PEE strongly affects RGC, neuronal migration and corticogenesis. These morphological alterations could be involved in the patophysiology of neurodevelopment disorders observed in the children of alcoholic mothers affected by the fetal alcohol syndrome. Grants PICT 31964, UBACYT M-072.