IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
BDNF regulates Translation thorugh mTOR/p70S6K signaling in memory consolidation of fear-motivated learning task
Autor/es:
SLIPCZUK LN; BEKINSCHTEIN P; KATCHE C; CAMMAROTA M; IZQUIERDO I; MEDINA JH
Lugar:
Santiago de Chile, Chile
Reunión:
Workshop; Neuronal Signaling and Plasicity; 2008
Institución organizadora:
IBRO
Resumen:
It is widely accepted that consolidation of long-term memory (LTM) requires mRNA translation, but little is known about the cellular mechanisms in the brain that regulate this process. Mammalian target of rapamycin (mTOR) is a key regulator of translational efficacy and capacity, nevertheless its upstream and downstream signaling in memory formation remains unknown. Here, we show that LTM formation of one-trial inhibitory avoidance (IA) in rats, a hippocampus-dependent fear-motivated learning task, requires mTOR during training and 3hs afterwards.Bilateral intra-CA1 infusion of rapamycin, a selective mTOR inhibitor, 15 min before, or 3 hs after training completely hinders IA LTM without affecting short-term memory (STM) retention. Blocking BDNF activity hinders mTOR activity produced by IA learning, and blocking BDNF or mTOR activity blocks GluR1 expression induced by learning in a synaptic terminals enriched subcellular fraction. Therefore, our findings indicate that the regulation of hippocampal mRNA translation has two windows of activity required for IA consolidation through BDNF/mTOR signaling.