IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Light modifies Sonic hedgehog protein profile
Autor/es:
MADANES, D.; FOSSER, N.S.; RIOS, H.; PAGANELLI, ALEJANDRA R.
Reunión:
Congreso; XXVII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2012
Resumen:
In vertebrates, the primary cilium is a microtubule-based organelle that extendsfrom the cell surface as a single, non-motile structure, and is present on mostcell types in embryonic and adult tissues. Photoreceptors are the unique cellulartype in the retina that possesses a primary cilium which is involved in themaintenance of the outer segment (OS) of cones and rods. The morphogensonic hedgehog (Shh) is essential for photoreceptor differentiation and retinalcell survival through development. In addition, Shh is expressed in the retinaduring postnatal growth but its function is unclear. It is well known that Shhpathway take place in the non-motile cilium. In this work we studied the Shhprotein response and acetylated tubulin that labels the cilium, in a model ofvisual hypostimulation, by immunohistochemistry and western blot analyses.We found differences both in Shh and tubulin proteins that indicate anepigenetic modulation of the OS by light. These results support our previoushypothesis that light modifies Shh protein profile, which in turn possibly couldmodify the cilium and therefore the outer segment. UBACYT 20020090200198