IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression of the Eph/ephrin system in chick retina regeneration
Autor/es:
DI NAPOLI, JENNIFER; MADRIGAL, A; ECHEVERRY NP; DEL RIO-TSONIS, KATIA; SCICOLONE, GABRIEL
Lugar:
Chicago
Reunión:
Congreso; Society for Developmental Biology (SDB) 70th Annual Meeting.; 2011
Institución organizadora:
Society for Developmental Biology
Resumen:
The embryonic chick has the capacity to regenerate its retina upon retinectomy via the activation of stem/progenitor cells in the anterior margin of the eye or by the transdifferentiation of the retinal pigmented epithelium (RPE). The optimal outcome of retinal regeneration is to obtain a functional retina. In this context, the Retinal Ganglion Cell (RGC) axons must reach the optic tectum (OT) and form the correct topographic map. Ephs and ephrins constitute the main molecular system involved in this process. The Eph receptor family consists of a group of transmembrane protein kinases that include two subgroups, the EphA and EphB receptors, depending on the type of ligands with which they interact. Ephrins can bind Eph receptors via their receptor binding domain. Ephrin-As are anchored to the membrane by Glycosylphosphatidylinositol whereas ephrin-Bs are transmembrane proteins. The Ephs and the ephrins are expressed in opposite gradients in the developing retina and the OT. The EphA/ephrinA system is involved in defining the topographic retinotectal connections along the rostrocaudal axis, whereas the EphB/ephrinB system is implicated in the dorsoventral axis and also in directing RGC axon path finding from their position in the retina to the Optic Disc (OD). In this study we analize the expression of the Eph/ephrin system in the developing and regenerating chick retina in order to establish if the missexpression of this system could be one of the reasons for the failure of the RGC axons of the transdifferentieted retina to reach the OD. For this purpose we performed immunohisto-chemistry against several Ephs and ephrins in developing and regenerated retinas at different developmental stages.