IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Identification of Axonal transport properties of VMAT2-YFP fluorescent vesicles
Autor/es:
CROMBERG L; OTERO MG; FALZONE T
Lugar:
Huerta Grande,
Reunión:
Congreso; XXVI Congreso de la Sociedad Argentina de Neurociencias (SAN); 2011
Institución organizadora:
SAN
Resumen:
Parkinsons disease
(PD) is characterized by reductions in dopamine neurotransmitter release, abnormal
protein accumulation known as Lewy Bodies, and selective degeneration of
dopamine neurons. Dopamine (DA) neurotransmission rely on ATP-dependent internalization
of DA by the Vesicular Monoamine Transporter 2 (VMAT2) prior to Ca-mediated
release. VMAT2 reductions in mice induce PD phenotypes and neurodegeneration
suggesting the relevance for the study of VMAT2 distribution along neurons. To identify
the axonal transport properties of VMAT2 we have generated a fusion to the
Yellow Fluorescent Protein (VMAT2-YFP). Initially, sucrose density flotation
experiments in transfected N2a cells revealed that VMAT-YFP floats associated
to vesicular membranes. Movies from transfected mouse primary hippocampal
neurons under live imaging experiments were transformed to kymographs for
analysis revealing a vesicular axonal transport for VMAT2-YFP with XX% of
anterograde, XX% of retrograde and XX% of stationary particles. Average speeds
for anterograde (XXum/sec) and retrograde (XXum/sec) vesicle displacements
correspond to motor dependent movement. These results suggest that VMAT2 axonal
transport is necessary for normal DA neurotransmission and implies a high
relevance for the identification of the motor moving VMAT2. In addition, VMAT2 axonal
transport defects might have a significant impact in the manifestation of PD
phenotypes and in the progression of DA neurodegeneration.