IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of prenatal exposure to cannabinoid agonist on postmitotic neuroblasts and glutamatergic cortical neurons in the developing cerebral cortex
Autor/es:
TMM. SAEZ; H. A. BRUSCO
Lugar:
Florencia
Reunión:
Congreso; 8TH IBRO WORLD CONGRESS OF NEUROSCIENCE; 2011
Institución organizadora:
International Brain Research Organization
Resumen:
Marihuana consumption during pregnancy represents a major risk for the developing fetus. The endocannabinoid system plays a modulatory role in specific processes of brain development such as radial and tangential migration and cell proliferation. Neurons of the cerebral cortex derive from two sources: projection neurons, which migrate radially from the neuroepithelium of the dorsal pallium and interneurons, which migrate tangentially from the ganglionic eminence. Cajal-Retzius (CR) cells are the earliest generated cortical neurons and migrate from the borders of the developing pallium. Doublecortin (DCX) is a microtubule-associated protein expressed in postmitotic neurons during periods of migration. Tbr1 is a transcription factor gene of the T box family that is expressed in Glutamatergic early-born cortical neurons. The aim of this study was to assess the effects of prenatal exposure to cannabinoid CB1/CB2 receptor agonist WIN55,212-2 (WIN) on distribution of postmitotic neuroblast and Glutamatergic cortical neurons, and number CR cells and cell proliferation in the fetal cerebral cortex. Pregnant Wistar rats were treated, from gestational day 5 to 16, at a daily s.c. WIN dose (0.75mg/kg). WIN-exposed rats showed altered distribution and orientation of tangential and radial migrating postmitotic neuroblast (DCX+) and also the radial distribution of postmitotic projections neurons (Tbr1+) along the Cortical Plate. No changes were observed in number of proliferating cells (Ki67+) in the ventricular zone. Prenatal exposure to WIN increase the number of CR cells (Reelin +) in the developing cortex. The effects of cannabinoids on cortical development may by due to its effects on the generation and/or migration of neuroblasts and postmitotic projection neurons. These findings could explain, at least in part, how prenatal exposure to cannabinoids can affect the developing CNS and induce behavioural and cognitive deficits frequently described in children and adolescents who´s mothers smoked marihuana during pregnancy