The role of PDLIM1, a PDZ‐LIM domain protein, at the ribbon synapses in the chicken retina

RÍOS, HUGO; PAGANELLI, ALEJANDRA R.; FOSSER, NICOLÁS S.

JOURNAL OF COMPARATIVE NEUROLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2020 vol. 528 p. 1820 - 1832

0021-9967

PDLIM´s protein family is involved in the rearrangement of the actin cytoskeleton. Inthe present study, we describe the localization of PDLIM1 in chicken photoreceptors.This study provides evidence that this protein is present at the cone pedicles, as wellas in other synapses of the chicken retina. Here, we demonstrate the expression patternof PDLIM1 through immunofluorescence staining, immunoblots, subcellular fractionation,and immunoprecipitation experiments. Also, we consider the possibilitythat PDLIM1 may be involved in the synaptic vesicle endocytosis and/or the presynaptictrafficking of synaptic vesicles back to the nonready releasable pool. This endocytotic/exocytotic coupling requires a tight link between exocytic vesicle fusion atdefined release sites and endocytic retrieval of synaptic vesicle membranes. In turn,photoreceptor ribbon synaptic structure depends on the cytoskeleton arrangement,both at the active zone-related with exocytosis?as well as at the endocytic zone?periactive zone. To our knowledge, the PDLIM1 protein has not been observed inthe pre synapses of the retina. Thus, the present study describes the expression andsubcellular localization of PDLIM1 for the first time, as well as its modulation byvisual environment in the chicken retina.