SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation

GATTI, CINTIA R.; ORQUEDA, ANDRÉS J.; FALZONE, TOMÁS L.; ORQUEDA, ANDRÉS J.; FALZONE, TOMÁS L.; OGARA, MARÍA F.; OGARA, MARÍA F.; GATTI, CINTIA R.

FEBS LETTERS

ELSEVIER SCIENCE BV

Año: 2018 vol. 592 p. 3708 - 3719

0014-5793

Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activity during neuronal differentiation. To study the dependence of LINE-1 activity on SOX-11 during neuronal differentiation, we induced differentiation of human SH-SY5Y neuroblastoma cells and adult adipose mesenchymal stem cells (hASCs) to a neuronal fate and found increased LINE-1 activity. We also show that SOX-11 protein binding to the LINE-1 promoter is higher in differentiating neuroblastoma cells, while knock-down of SOX-11 inhibits the induction of LINE-1 transcription in differentiating conditions. These results suggest that activation of LINE-1 retrotransposition during neuronal differentiation is mediated by SOX-11.