IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures
Autor/es:
IGNACIO M. LARRÁYOZ, PHD; MANUEL REY-FUNES; DANIELA S CONTARTESE; FEDERICO ROLÓN; ANIBAL SAROTTO; VERONICA B DORFMAN; CESAR F LOIDL; ALFREDO MARTINEZ; IGNACIO M. LARRÁYOZ, PHD; MANUEL REY-FUNES; DANIELA S CONTARTESE; FEDERICO ROLÓN; ANIBAL SAROTTO; VERONICA B DORFMAN; CESAR F LOIDL; ALFREDO MARTINEZ
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2016
ISSN:
1932-6203
Resumen:
Hypothermia has been proposed as a therapeutic intervention for some retinal conditions,including ischemic insults. Cold exposure elevates expression of cold-shock proteins(CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein(CIRP), but their presence in mammalian retina is so far unknown. Here we show theeffects of hypothermia on the expression of these CSPs in retina-derived cell lines and inthe retina of newborn and adult rats. Two cell lines of retinal origin, R28 and mRPE, wereexposed to 32°C for different time periods and CSP expression was measured by qRTPCRand Western blotting. Neonatal and adult Sprague-Dawley rats were exposed to acold environment (8°C) and expression of CSPs in their retinas was studied by Westernblotting, multiple inmunofluorescence, and confocal microscopy. RBM3 expression wasupregulated by cold in both R28 and mRPE cells in a time-dependent fashion. On the otherhand, CIRP was upregulated in R28 cells but not in mRPE. In vivo, expression of CSPs wasnegligible in the retina of newborn and adult rats kept at room temperature (24°C). Exposureto a cold environment elicited a strong expression of both proteins, especially in retinal pigmentepithelium cells, photoreceptors, bipolar, amacrine and horizontal cells, Müller cells,and ganglion cells. In conclusion, CSP expression rapidly rises in the mammalian retina followingexposure to hypothermia in a cell type-specific pattern. This observation may be atthe basis of the molecular mechanism by which hypothermia exerts its therapeutic effects inthe retina.