IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
Nitric Oxide System Alteration at Spinal Cord as a Result of Perinatal Asphyxia IsInvolved in Behavioral Disabilities: Hypothermia as Preventive Treatment
Autor/es:
VERONICA BERTA DORFMAN, MANUEL REY-FUNES, JULIO CESAR BAYONA, ESTER MARÝA LOPEZ, HECTOR COIRINI, AND CESAR FABIAN LOIDL
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Editorial:
Wiley Interscience
Referencias:
Lugar: United States; Año: 2008 vol. 87 p. 1260 - 1269
ISSN:
0360-4012
Resumen:
Perinatal asphyxia (PA) is able to induce sequelae such
as spinal spasticity. Previously, we demonstrated hypothermia
as a neuroprotective treatment against cell
degeneration triggered by increased nitric oxide (NO)
release. Because spinal motoneurons are implicated in
spasticity, our aim was to analyze the involvement of
NO system at cervical and lumbar motoneurons after
PA as well as the application of hypothermia as treatment.
PA was performed by immersion of both uterine
horns containing full-term fetuses in a water bath at
378C for 19 or 20 min (PA19 or PA20) or at 158C for 20
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.
8C for 19 or 20 min (PA19 or PA20) or at 158C for 20
min (hypothermia during PA-HYP). Some randomly chosen
PA20 rats were immediately exposed for 5 min
over grain ice (hypothermia after PA-HPA). Full-term
vaginally delivered rats were used as control (CTL). We
analyzed NO synthase (NOS) activity, expression and
localization by nicotinamide adenine dinucleotide phosphate
diaphorase (NADPH-d) reactivity, inducible and
neuronal NOS (iNOS and nNOS) by immunohistochemistry,
and protein nitrotyrosilation state. We observed an
increased NOS activity at cervical spinal cord of 60-
day-old PA20 rats, with increased NADPH-d, iNOS, and
nitrotyrosine expression in cervical motoneurons and
increased NADPH-d in neurons of layer X. Lumbar neurons
were not altered. Hypothermia was able to maintain
CTL values. Also, we observed decreased forelimb
motor potency in the PA20 group, which could be
attributed to changes at cervical motoneurons. This
study shows that PA can induce spasticity produced by
alterations in the NO system of the cervical spinal cord.
Moreover, this situation can be prevented by perinatal
hypothermia. VVC 2008 Wiley-Liss, Inc.VVC 2008 Wiley-Liss, Inc.