IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
The NMDAR Subunit NR2B Expression is Modified in Hippocampus after Repetitive Seizures
Autor/es:
AUZMENDI, GONZÁLEZ, GIRARDI
Revista:
NEUROCHEMICAL RESEARCH
Editorial:
Springer
Referencias:
Lugar: New York; Año: 2008 vol. 34
ISSN:
0364-3190
Resumen:
Abstract NMDA receptor is involved in synaptic plasticity,
learning, memory and neurological diseases like
epilepsia and it is the major mediator of excitotoxicity.
NR2B-containing NMDA receptors may be playing a
crucial role in epileptic disorders. In the present study the
effect of the convulsant drug 3-mercaptopropionic acid
(MP) repetitive administration (47 days) on the hippocampal
NR2B subunit was studied. A significant decrease
in NR2B in the whole hippocampus was observed after
MP4 with a tendency to recover to normal values in MP7
by western blot assay. Immunohistochemical studies
showed a decrease in several CA1 and CA2/3 strata (21
73%). MP7 showed a reversion of the drop observed at
4 days in stratum oriens, pyramidal cell layer in CA1,
CA2/3 and CA1 stratum radiatum. A significant fall in the
lacunosum molecular layer of both areas and stratum radiatum
of CA2/3 was observed. The immunostaining in
MP4 showed a decrease in the granulare layer from dentate
gyrus (20%), in hillus (71%) and subicullum (63%) as
compared with control and these decreases were similar at
MP7 values. Results showed decreases in NR2B subunit
expression in different areas following repeated MP-induce
seizures, suggesting that NR2B expression is altered
depending on the diverse hippocampal input and output
signals of each region that could be differently involved in
modulating MP-induced hyperactivity.
learning, memory and neurological diseases like
epilepsia and it is the major mediator of excitotoxicity.
NR2B-containing NMDA receptors may be playing a
crucial role in epileptic disorders. In the present study the
effect of the convulsant drug 3-mercaptopropionic acid
(MP) repetitive administration (47 days) on the hippocampal
NR2B subunit was studied. A significant decrease
in NR2B in the whole hippocampus was observed after
MP4 with a tendency to recover to normal values in MP7
by western blot assay. Immunohistochemical studies
showed a decrease in several CA1 and CA2/3 strata (21
73%). MP7 showed a reversion of the drop observed at
4 days in stratum oriens, pyramidal cell layer in CA1,
CA2/3 and CA1 stratum radiatum. A significant fall in the
lacunosum molecular layer of both areas and stratum radiatum
of CA2/3 was observed. The immunostaining in
MP4 showed a decrease in the granulare layer from dentate
gyrus (20%), in hillus (71%) and subicullum (63%) as
compared with control and these decreases were similar at
MP7 values. Results showed decreases in NR2B subunit
expression in different areas following repeated MP-induce
seizures, suggesting that NR2B expression is altered
depending on the diverse hippocampal input and output
signals of each region that could be differently involved in
modulating MP-induced hyperactivity.
learning, memory and neurological diseases like
epilepsia and it is the major mediator of excitotoxicity.
NR2B-containing NMDA receptors may be playing a
crucial role in epileptic disorders. In the present study the
effect of the convulsant drug 3-mercaptopropionic acid
(MP) repetitive administration (47 days) on the hippocampal
NR2B subunit was studied. A significant decrease
in NR2B in the whole hippocampus was observed after
MP4 with a tendency to recover to normal values in MP7
by western blot assay. Immunohistochemical studies
showed a decrease in several CA1 and CA2/3 strata (21
73%). MP7 showed a reversion of the drop observed at
4 days in stratum oriens, pyramidal cell layer in CA1,
CA2/3 and CA1 stratum radiatum. A significant fall in the
lacunosum molecular layer of both areas and stratum radiatum
of CA2/3 was observed. The immunostaining in
MP4 showed a decrease in the granulare layer from dentate
gyrus (20%), in hillus (71%) and subicullum (63%) as
compared with control and these decreases were similar at
MP7 values. Results showed decreases in NR2B subunit
expression in different areas following repeated MP-induce
seizures, suggesting that NR2B expression is altered
depending on the diverse hippocampal input and output
signals of each region that could be differently involved in
modulating MP-induced hyperactivity.
learning, memory and neurological diseases like
epilepsia and it is the major mediator of excitotoxicity.
NR2B-containing NMDA receptors may be playing a
crucial role in epileptic disorders. In the present study the
effect of the convulsant drug 3-mercaptopropionic acid
(MP) repetitive administration (47 days) on the hippocampal
NR2B subunit was studied. A significant decrease
in NR2B in the whole hippocampus was observed after
MP4 with a tendency to recover to normal values in MP7
by western blot assay. Immunohistochemical studies
showed a decrease in several CA1 and CA2/3 strata (21
73%). MP7 showed a reversion of the drop observed at
4 days in stratum oriens, pyramidal cell layer in CA1,
CA2/3 and CA1 stratum radiatum. A significant fall in the
lacunosum molecular layer of both areas and stratum radiatum
of CA2/3 was observed. The immunostaining in
MP4 showed a decrease in the granulare layer from dentate
gyrus (20%), in hillus (71%) and subicullum (63%) as
compared with control and these decreases were similar at
MP7 values. Results showed decreases in NR2B subunit
expression in different areas following repeated MP-induce
seizures, suggesting that NR2B expression is altered
depending on the diverse hippocampal input and output
signals of each region that could be differently involved in
modulating MP-induced hyperactivity.
learning, memory and neurological diseases like
epilepsia and it is the major mediator of excitotoxicity.
NR2B-containing NMDA receptors may be playing a
crucial role in epileptic disorders. In the present study the
effect of the convulsant drug 3-mercaptopropionic acid
(MP) repetitive administration (47 days) on the hippocampal
NR2B subunit was studied. A significant decrease
in NR2B in the whole hippocampus was observed after
MP4 with a tendency to recover to normal values in MP7
by western blot assay. Immunohistochemical studies
showed a decrease in several CA1 and CA2/3 strata (21
73%). MP7 showed a reversion of the drop observed at
4 days in stratum oriens, pyramidal cell layer in CA1,
CA2/3 and CA1 stratum radiatum. A significant fall in the
lacunosum molecular layer of both areas and stratum radiatum
of CA2/3 was observed. The immunostaining in
MP4 showed a decrease in the granulare layer from dentate
gyrus (20%), in hillus (71%) and subicullum (63%) as
compared with control and these decreases were similar at
MP7 values. Results showed decreases in NR2B subunit
expression in different areas following repeated MP-induce
seizures, suggesting that NR2B expression is altered
depending on the diverse hippocampal input and output
signals of each region that could be differently involved in
modulating MP-induced hyperactivity.
NMDA receptor is involved in synaptic plasticity,
learning, memory and neurological diseases like
epilepsia and it is the major mediator of excitotoxicity.
NR2B-containing NMDA receptors may be playing a
crucial role in epileptic disorders. In the present study the
effect of the convulsant drug 3-mercaptopropionic acid
(MP) repetitive administration (47 days) on the hippocampal
NR2B subunit was studied. A significant decrease
in NR2B in the whole hippocampus was observed after
MP4 with a tendency to recover to normal values in MP7
by western blot assay. Immunohistochemical studies
showed a decrease in several CA1 and CA2/3 strata (21
73%). MP7 showed a reversion of the drop observed at
4 days in stratum oriens, pyramidal cell layer in CA1,
CA2/3 and CA1 stratum radiatum. A significant fall in the
lacunosum molecular layer of both areas and stratum radiatum
of CA2/3 was observed. The immunostaining in
MP4 showed a decrease in the granulare layer from dentate
gyrus (20%), in hillus (71%) and subicullum (63%) as
compared with control and these decreases were similar at
MP7 values. Results showed decreases in NR2B subunit
expression in different areas following repeated MP-induce
seizures, suggesting that NR2B expression is altered
depending on the diverse hippocampal input and output
signals of each region that could be differently involved in
modulating MP-induced hyperactivity.