IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
Functional Expression of Brain Neuronal CB2 Cannabinoid Receptors Are Involved in the Effects of Drugs of Abuse and in Depression
Autor/es:
ONAIVI E, ISHIGURO H, GONG JP, PATEL S, MEOZZI PA, MYERS L, PERCHUK A, MORA Z, TAGLIAFERRO P, GARDNER E, BRUSCO A, AKINSHOLA BM, LIU QR, SHIRWA SS; HOPE B, LUJILDE J, INADA T, IWASAKI S, MACHARIA D, TEASENFITZ L, ARINAMI T, UHL GR
Revista:
Annals of the New York Academy of Science
Editorial:
Blackwell Publishing o behalf of the New York Academy of Sciences
Referencias:
Lugar: Boston, Massachusetts, USA; Año: 2008 vol. 1139 p. 434 - 439
ISSN:
0077-8923
Resumen:
Major depression and addiction are mental health problems associated with stressful
events in life with high relapse and recurrence even after treatment. Many laboratories
were not able to detect the presence of CB2 cannabinoid receptors (CB2-Rs) in healthy
brains, but CB2-R expression has been demonstrated in rat microglial cells and other
brain-associated cells during inflammation. Thus, neuronal expression of CB2-Rs has
been ambiguous and controversial, and its role in depression and substance abuse is
unknown. In this study we tested the hypothesis that genetic variants of the CB2 gene
might be associated with depression in a human population and that alteration in CB2CB2 gene
might be associated with depression in a human population and that alteration in CB2CB2
gene expression may be involved in the effects of abused substances, including opiates,
cocaine, and ethanol, in rodents. Here we demonstrate that a high incidence of Q63R
but not H316Y polymorphism in the CB2 gene was found in Japanese depressed subjects.
CB2-Rs and their gene transcripts are expressed in the brains of na¨ývemice and are
modulated after exposure to stressors and administration of abused drugs.Mice that developed
an alcohol preference had reduced CB2 gene expression, and chronic treatment
with JWH015 a putative CB2-R agonist, enhanced alcohol consumption in stressed but
not in control mice. The direct intracerebroventricularmicroinjection of CB2 antisense
oligonucleotide into the mouse brain reduced mouse aversions in the plus-maze test,
indicating the functional presence of CB2-Rs in the brain that modifies behavior. Using
electron microscopy we report the subcellular localization of CB2-Rs that are mainly
on postsynaptic elements in rodent brain. Our data demonstrate the functional expression
of CB2-Rs in the brain that may provide novel targets for the effects of cannabinoids
in depression and substance abuse disorders beyond neuroimmunocannabinoid
activity
on postsynaptic elements in rodent brain. Our data demonstrate the functional expression
of CB2-Rs in the brain that may provide novel targets for the effects of cannabinoids
in depression and substance abuse disorders beyond neuroimmunocannabinoid
activityCB2 gene was found in Japanese depressed subjects.
CB2-Rs and their gene transcripts are expressed in the brains of na¨ývemice and are
modulated after exposure to stressors and administration of abused drugs.Mice that developed
an alcohol preference had reduced CB2 gene expression, and chronic treatment
with JWH015 a putative CB2-R agonist, enhanced alcohol consumption in stressed but
not in control mice. The direct intracerebroventricularmicroinjection of CB2 antisense
oligonucleotide into the mouse brain reduced mouse aversions in the plus-maze test,
indicating the functional presence of CB2-Rs in the brain that modifies behavior. Using
electron microscopy we report the subcellular localization of CB2-Rs that are mainly
on postsynaptic elements in rodent brain. Our data demonstrate the functional expression
of CB2-Rs in the brain that may provide novel targets for the effects of cannabinoids
in depression and substance abuse disorders beyond neuroimmunocannabinoid
activity
on postsynaptic elements in rodent brain. Our data demonstrate the functional expression
of CB2-Rs in the brain that may provide novel targets for the effects of cannabinoids
in depression and substance abuse disorders beyond neuroimmunocannabinoid
activityCB2 gene expression, and chronic treatment
with JWH015 a putative CB2-R agonist, enhanced alcohol consumption in stressed but
not in control mice. The direct intracerebroventricularmicroinjection of CB2 antisense
oligonucleotide into the mouse brain reduced mouse aversions in the plus-maze test,
indicating the functional presence of CB2-Rs in the brain that modifies behavior. Using
electron microscopy we report the subcellular localization of CB2-Rs that are mainly
on postsynaptic elements in rodent brain. Our data demonstrate the functional expression
of CB2-Rs in the brain that may provide novel targets for the effects of cannabinoids
in depression and substance abuse disorders beyond neuroimmunocannabinoid
activity
on postsynaptic elements in rodent brain. Our data demonstrate the functional expression
of CB2-Rs in the brain that may provide novel targets for the effects of cannabinoids
in depression and substance abuse disorders beyond neuroimmunocannabinoid
activity