IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
Anaglyph of Retinal Stem Cells and Developing Axons: Selective Volume Enhancement in Microscopy Images
Autor/es:
CARRI, NÉSTOR GABRIEL; BERMÚDEZ, SN; FIORE, LUCIANO; DI NAPOLI, JENNIFER; SCICOLONE, GABRIEL
Revista:
The Anatomical Record
Editorial:
John Wiley & Sons, Inc.
Referencias:
Año: 2014 vol. 297 p. 770 - 780
ISSN:
1932-8494
Resumen:
Retinal stem cell culture has become a powerful research tool, but itrequires reliable methods to obtain high-quality images of living and fixedcells. This study describes a procedure for using phase contrast microscopyto obtain three-dimensional (3-D) images for the study of living cellsby photographing a living cell in a culture dish from bottom to top, aswell as a procedure to increase the quality of scanning electron micrographsand laser confocal images. The procedure may also be used to photographclusters of neural stem cells, and retinal explants with vigorousaxonal growth. In the case of scanning electron microscopy and laser confocalimages, a Gaussian procedure is applied to the original images. Themethodology allows for the creation of anaglyphs and video reconstructions,and provides high-quality images for characterizing living cells ortissues, fixed cells or tissues, or organs observed with scanning electronand laser confocal microscopy. Its greatest advantage is that it is easy toobtain good results without expensive equipment. The procedure is fast,precise, simple, and offers a strategic tool for obtaining 3-D reconstructionsof cells and axons suitable for easily determining the orientationand polarity of a specimen. It also enables video reconstructions to be created,even of specimens parallel to the plastic base of a tissue culturedish, It is also helpful for studying the distribution and organization ofliving cells in a culture, as it provides the same powerful information asoptical tomography, which most confocal microscopes cannot do on sterileliving cells. Anat Rec, 00:000?000, 2014. VC 2014 Wiley Periodicals, Inc.