IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
CHANGES IN CENTRAL NERVOUS SYSTEM CELLS IN HYPERAMMONEMIC PORTAL HYPERTENSIVE RATS
Autor/es:
SILVINA TALLIS; LAURA CALTANA; PABLO SOUTO; AMALIA DELFANTE; ALICIA BRUSCO; JUAN PERAZZO
Revista:
JOURNAL OF NEUROCHEMISTRY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2014 vol. 128 p. 431 - 444
ISSN:
0022-3042
Resumen:
Rats with prehepatic portal hypertension due to partial portal vein ligation (PVL) developed hyperammonemia, impaired blood-brain barrier, mild brain edema and severe mitochondrial changes in hippocampus. The aim of this study was to evaluate changes of different neural cells in brain cortex and hippocampus. Animals were divided in two groups, PVL and sham. Astrocytes were studied by immunostaining with GFAP and S100β protein; neurons with NeuN, MAP-2 and NF-200 and capillaries with Nestin. The hypoxia-inducible factor 1α (HIF-1α) and its downstream proteins, Pglycoprotein (P-gp) and erythropoietin receptor (Epo-R) were also evaluated. Astrocytes were increased in area and number only in the hippocampus, meanwhile S100 β increased in both brain areas in PVL animals. MAP-2 and NF-200 immunoreactivity (-ir) were significantly reduced in both areas. Hippocampal Nestin-ir was increased in PVL animals. These cellular changes were similar to those described in ischemic conditions, so that HIF-1α, P-gp and Epo-R were also evaluated. A high expression of HIF-1α in cortical neurons in PVL group was observed. It could be proposed that this hypoxia-like state is triggered via ammonia by occupying the binding domain of HIF-1α, thereby preventing its degradation and inducing its stabilization leading to theoverexpression of P-gp and the Epo-R.