IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
Attenuated response to methanphetamine sensitization and deficits in motor learning and memory after selective deletion of beta catenin in dopamine neurons.
Autor/es:
DIAZ-RUIZ, OSCAR; ZHANG, YAJUN; SHAN, LUFEI ; MALIK, NASIR; HOFFMAN, ALEXANDER F; CADET, JEAN LUD; LUPICA, CARL R; TAGLIAFERRO, ADRIANA; BRUSCO, ALICIA; BACKMAN, CRISTINA
Revista:
NEUROBIOLOGY OF LEARNING AND MEMORY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 19 p. 341 - 350
ISSN:
1074-7427
Resumen:
In the present study, we analyzed mice with a targeted deletion of b-catenin in DA neurons (DA-bcat KO mice) to address the functional significance of this molecule in the shaping of synaptic responses associated with motor learning and following exposure to drugs of abuse. Relative to controls, DA-bcat KO mice showed significant deficits in their ability to formlong-term memories and displayed reduced expression of methamphetamine-induced behavioral sensitization after subsequent challenge doses with this drug, suggesting that motor learning and drug-induced learning plasticity are altered in these mice. Morphological analyses showed no changes in the number or distribution of tyrosine hydroxylase-labeled neurons in the ventral midbrain. While electrochemical measurements in the striatum determined no changes in acuteDA release and uptake, a small but significant decrease in DA release was detected in mutant animals after prolonged repetitive stimulation, suggesting a possible deficit in the DA neurotransmitter vesicle reserve pool. However, electron microscopy analyses did not reveal significant differences in the content of synaptic vesicles per terminal, and striatal DA levels were unchanged in DA-bcat KO animals. In contrast, striatal mRNA levels for several markers known to regulate synaptic plasticity and DA neurotransmission were altered in DA-bcat KO mice. This study demonstrates that ablation of b-catenin in DA neurons leads to alterations of motor and reward-associated memories and to adaptations of the DA neurotransmitter system and suggests that b-catenin signaling in DA neurons is required to facilitate the synaptic remodeling underlyingthe consolidation of long-term memories.