IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
Adverse Effects of 2,4-Dichlorophenoxyacetic Acid on Rat Cerebellar Granule Cell Cultures Were Attenuated by Amphetamine
Autor/es:
BONGIOVANNI B; FERRI A; BRUSCO A; RASSETTO M; LOPEZ LM; EVANGELISTA DE DUFFARD AM; DUFFARD R
Revista:
NEUROTOXICITY RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: New York; Año: 2011 vol. 19 p. 544 - 555
ISSN:
1029-8428
Resumen:
2,4-Dichlorophenoxyacetic acid (2,4-D), a
worldwide-used herbicide, has been shown to produce a
wide range of adverse effects in the health?from embryotoxicity
and teratogenicity to neurotoxicity?of animals
and humans. In this study, neuronal morphology and biochemical
events in rat cerebellar granule cell (CGC) cultures
have been analyzed to define some of the possible
mechanisms involved in 2,4-D-induced cell death. For that
purpose, amphetamine (AMPH) that has been shown to
accelerate the recovery of several functions in animals with
brain injury has been used as a pharmacologycal tool and
was also investigated as a possible protecting agent.
Addition of 2,4-D to CGC cultures produced a drastic
decrease in cell viability, in association with an increased
incidence of necrosis and apoptosis, and an increased level
of reactive oxygen species, a decrease in glutathione content,
and an abnormal activity of some enzymes with
respect to the control group. The adverse effects of 2,4-D
were partly attenuated in presence of AMPH. Some deleterious
effects on several ultrastructural features of the cells, as
well as the enhanced incidence of apoptosis, were partially
preserved in AMPH-protected cultures as compared with
those which were exposed to 2,4-D alone. The collected
evidences (1) confirms the previously observed, deleterious
effects of 2.4D on the same or a similar model; (2) suggests
that the 2,4-D-induced apoptosis could have been mediated
by or associated to an oxidative imbalance in the affected
cells, and (3) shows some evidence of a protective effect of
AMPH on 2,4-D-induced cell death, which could have been
exerted through a reduction in the oxidative stress.