IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
3-mercaptopropionic acid induced seizures decrease NR2B expression in Purkinje cells. Cyclopentyladenosine effect.
Autor/es:
E. GIRARDI J. AUZMENDI N. CHARO´
Revista:
CELLULAR AND MOLECULAR NEUROBIOLOGY.
Editorial:
SPRINGER/PLENUM PUBLISHERS
Referencias:
Lugar: USA; Año: 2010 vol. 30 p. 985 - 990
ISSN:
0272-4340
Resumen:
Abstract Inhibitory mechanism of cerebellum epileptic
activity can be involved depending on the intensity and
frequency of seizure convulsions. N-methyl-D-aspartate
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
activity can be involved depending on the intensity and
frequency of seizure convulsions. N-methyl-D-aspartate
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
activity can be involved depending on the intensity and
frequency of seizure convulsions. N-methyl-D-aspartate
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
Inhibitory mechanism of cerebellum epileptic
activity can be involved depending on the intensity and
frequency of seizure convulsions. N-methyl-D-aspartate
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
N-methyl-D-aspartate
receptors (NMDARs) play key roles in excitatory synaptic
transmission and have been implicated in neurological
disorders: in cerebellum, they have specific characteristics.
NMDARs are heteromeric complexes, and the expression
of functional receptors in mammalian cells requires the
subunit NR1 (essential) and one NR2 subtype of the
four isoforms: NR2ANR2D. In mature Purkinje cells, the
combination of NR1 with NR2B subunits forms functional
NMDARs; NR2B subunit may be altered in exocitotoxic
events. Cyclopentyladenosine (CPA), an adenosine analogue,
administered to rats, for one or more days, increases
seizure threshold induced by the convulsant drug 3-mercaptopropionic
acid (MP). In this study, we focused on the
expression of NR2B in cerebellum after repetitive seizures
induced by MP and the effect of adenosine analogue CPA
administered alone or previous to MP (CPA ? MP). A
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observed after MP and CPA administration with a tendency
to recover to normal values in the combined treatment of
CPA administered 30 min before MP by Western blot
assay. In immunohistochemical studies, NR2B expression
was observed and analysed in Purkinje cells. NR2B
expression was decreased after MP (55%) and CPA (12%)
administration, and CPA injected 30 min before MP led to
28% reduction in Purkinje cells. These results could be
related to Purkinje cell damage or alternatively to avoid the
excitotoxic effect. Results recorded after CPA + MP
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
treatment seemed involved in decreasing the convulsant
MP effect
significant decrease in NR2B in the whole cerebellum was
observ