Differential Immune Response of Lactobacillus plantarum 286 Against Salmonella Typhimurium Infection in Conventional and Germ-Free Mice

ORNELLAS, ROBERTA MARIA DOS SANTOS; DA COSTA, ANDRÉA MIURA; VINDEROLA, GABRIEL; ACURCIO, LEONARDO BORGES; UETANABARO, ANA PAULA TROVATTI; SANTOS, TIZÁ TELES; SANDES, SÁVIO HENRIQUE CICCO; NICOLI, JACQUES ROBERT

ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY.

SPRINGER-VERLAG BERLIN

Año: 2020

0065-2598

We aimed at evaluating in vivo the probioticpotential of Lactobacillus plantarum286 against Salmonella enterica serov.Typhimurium. Colonization capacity andantagonistic activity were determined in fecesof gnotobiotic mice. Survival to infection,translocation, histopathology, IgA and cytokinelevels (IL-10, IL-6, IFN-γ, TNF-α,TGF-β) were determined both in conventionaland germ-free mice followed L. plantarum286 administration and Salmonella infection.L. plantarum 286 colonized the intestine ofgnotobiotic mice, where it produced antagonisticsubstances against S. Typhimurium. Inconventional animals, the administration ofthis strain increased intestinal IgA levels andreduced the inflammatory response and thetissue damage caused by S. Typhimurium.Reduction of tissue damage in the intestineand liver of germ-free animals was alsoobserved, however the immune responseelicited was different in either model.L. plantarum 286 showed in vivo probioticproperties in both murine models. Probioticcapacity results may depend on the animalmodel chosen.