INLAIN   20354
INSTITUTO DE LACTOLOGIA INDUSTRIAL
Unidad Ejecutora - UE
artículos
Título:
Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition
Autor/es:
VIRGINIA BATISTELA; SOHAIB SADIQ; LUCIANA VERA CANDIOTI; ELISA C. ALE; JOANA B. FERRADO; JORGE A. REINHEIMER; ANA G. BINETTI; GABRIELA CORREA OLIVAR; HAFIZ AHMED; ANDREW P. LAWS
Revista:
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2020
ISSN:
1364-727X
Resumen:
Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β-glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semi-defined medium (SDM, 0.63% yeast nitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterization indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β-glucan.Keywords: exopolysaccharides; statistical optimisation; chemical composition, L. fermentum; response surface methodology.