Variability in gut mucosal secretory IgA in mice along a working day

BURNS, P.; TABACMAN, E.; ODDI, S.; REINHEIMER, J.; FORZANI, L.; VINDEROLA, G.

BMC Research Notes

BioMed Central

Año: 2018 vol. 11 p. 1 - 5

1756-0500

Objective: to assess the variability of S-IgA in the lumen and feces of mice along aworking day.Results: mice were maintained under a 12 h light - dark cycle, where the light periodstarted at 8 AM. S-IgA was determined in feces and in the intestinal content (after oneor three washes) at 3 points along a working day: at the beginning, in the middle and atthe end of the light period (named BLP, MLP, ELP). A significant reduction in thecontent of S-IgA in the small intestine fluid and in feces was observed at the end of thelight cycle, which coincides with the end of a regular working day (8 PM) in any givenanimal facility. It was also observed that 3 washes of the small intestine were moreeffective than one flush to recover a significant higher amount of S-IgA, with thesmallest CV observed by the ELP. A smaller CV would imply a reduced number ofanimals needed to achieve the same meaningful results. The results may be usefulwhen designing animal trails for the selection of probiotic candidates based on theircapacity of activating S-IgA, for a more rational use of experimental animals.