Isolation, characterisation and performance of autochthonous spray dried lactic acid bacteria in maize micro-silos.

BURNS, P.; PUNTILLO, M.; MAZZONI, R.; BURNS, P.; BINETTI, A. G.; PUNTILLO, M.; PÁEZ, R.; VINDEROLA, C. G.; MAZZONI, R.; BINETTI, A. G.; BORGO, M. F.; PÁEZ, R.; BERGAMINI, C.; VINDEROLA, C. G.; REINHEIMER, J. A.; BORGO, M. F.; BERGAMINI, C.; REINHEIMER, J. A.

Frontiers in Microbiology

Frontiers Media S.A.

Año: 2018

1664-302X

The aim of this study was to isolate, identify and characterize lactic acid bacteria (LAB) from spontaneously fermented corn silage, and to assess their performance as spray-dried (SD) cultures in corn micro-silos. Eleven isolates of presumptive LAB were obtained. The growth kinetics, the capacity to grow in vegetable-based medium (VBM) and to produce organic acids and the tolerance to heat-stress were determined. Lactobacillus plantarum Ls71, Pediococcus acidilactici Ls72 and Lactobacillus buchneri Ls141 were selected for further studies. For these strains, the capacity to grow in MRS and VBM, the resistance to freeze and spray-drying and the performance as SD bacteria in corn micro-silos were evaluated. In inoculated micro-silos a significantly lower pH was found after 4 d of fermentation when compared with controls. A significantly lower cell load of filamentous fungi and yeasts (< 3 log10 cfu/g) and a higher concentration of total LAB (> 8.7 log10 cfu/g) was observed after 30 d of fermentation (no yeasts and filamentous fungi were detected after 60 d). The selected strains have the potential to be produced as SD silage inoculant as they were able to accelerate the fermentation process, to control filamentous fungi and yeasts and to improve some nutritional and chemical parameters of silage.