INLAIN   20354
INSTITUTO DE LACTOLOGIA INDUSTRIAL
Unidad Ejecutora - UE
artículos
Título:
Phage resistance linked to cell heterogeneity in the commercial strain Lactobacillus delbrueckii subsp. lactis Ab1
Autor/es:
VIVIANA SUÁREZ; NATALIA MACIEL; DANIELA GUGLIELMOTTI; MIRIAM ZAGO; GIORGIO GIRAFFA; JORGE REINHEIMER
Revista:
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Editorial:
Wiley-Blackwell
Referencias:
Año: 2008 vol. 128 p. 401 - 401
ISSN:
0168-1605
Resumen:
The aim of this work was to study the relationship between the cell morphological heterogeneity and the phage-resistance in the commercial strain Lactobacillus delbrueckii subsp. lactis Ab1. Two morphological variants (named C and T) were isolated from this strain. Phage-resistant derivatives were isolated from them and the percentage of occurrence of confirmed phage-resistant cellswas 0.001% of the total cellular population. Within these phage-resistant cell derivatives there were T (3 out of 4 total isolates) and C (1 out of 4 total isolates) variants. The study of some technological properties (e.g. proteolytic and acidifying activities) demonstrated that most of phage-resistant derivatives were not as good as the parental strain. However, for one derivative (a T variant), the technological properties were better than those of the parental strain. On the other hand, it was possible to determinate that the systemof phage-resistance in the T variantswas interference in adsorption step, with adsorption rates b15%. For the C variant derivative it was possible to demonstrate the presence of a restriction/modification systemand,moreover, to determinate that this systemcould be Type I R/M.Lactobacillus delbrueckii subsp. lactis Ab1. Two morphological variants (named C and T) were isolated from this strain. Phage-resistant derivatives were isolated from them and the percentage of occurrence of confirmed phage-resistant cellswas 0.001% of the total cellular population. Within these phage-resistant cell derivatives there were T (3 out of 4 total isolates) and C (1 out of 4 total isolates) variants. The study of some technological properties (e.g. proteolytic and acidifying activities) demonstrated that most of phage-resistant derivatives were not as good as the parental strain. However, for one derivative (a T variant), the technological properties were better than those of the parental strain. On the other hand, it was possible to determinate that the systemof phage-resistance in the T variantswas interference in adsorption step, with adsorption rates b15%. For the C variant derivative it was possible to demonstrate the presence of a restriction/modification systemand,moreover, to determinate that this systemcould be Type I R/M.