Mortality and translocation assay to study the protective capacity of Bifidobacterium lactis INL-1 against Salmonella Typhimurium infection in mice

M.F.ZACARIAS; J. REINHEIMER; L.FORZANI; C. GRANGETTE; G. VINDEROLA

Beneficial Microbes

Wageningen Academic Publishers

Año: 2014 vol. 5 p. 427 - 436

1876-2883

The double use of cheese whey (culture medium and thermoprotectant for spray drying oflactobacilli) was explored in this study for adding value to this wastewater. In-house formulated broth(similar to MRS) and dairy media (cheese and ricotta whey and whey permeate) were assessed fortheir capacity to produce biomass of Lactobacillus paracasei JP1, Lb. rhamnosus 64 and Lb. gasseri37. Simultaneously, spray drying of cheese whey-starch solution (without lactobacilli cells) wasoptimised using surface response methodology. Cell suspensions of the lactobacilli, produced in inhouse-formulated broth, were spray-dried in cheese whey-starch solution and viability monitoredthroughout the storage of powders for 2 months. Lb. rhamnosus 64 was able to growsatisfactorily in atleast two of the in-house formulated culture media and in the dairy media assessed. It also performedwell in spray drying. The performance of the other strains was less satisfactory. The growth capacity,the resistance to spray drying in cheese whey-starch solution and the negligible lost in viability duringthe storage (2 months), makes Lb. rhamnosus 64 a promising candidate for further technologicalstudies for developing a probiotic dehydrated culture for foods, utilising wastewaters of the dairyindustry (as growth substrate and protectant) and spray drying (a low-cost widely-availabletechnology).