CHARACTERISATION OF THREE TEMPERATE PHAGES RELEASED FROM THE SAME LACTOBACILLUS PARACASEI COMMERCIAL STRAIN

CAPRA, MARÍA LUJÁN; MERCANTI, DIEGO JAVIER; REINHEIMER, JORGE ALBERTO; QUIBERONI, ANDREA DEL LUJÁN

INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2010 vol. 63 p. 396 - 405

1364-727X

<!-- /* Font Definitions */ @font-face {font-family:AdvTT5843c571; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"Arial Unicode MS"; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:1 134676480 16 0 131072 0;} @font-face {font-family:"@AdvTT5843c571"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:1 134676480 16 0 131072 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Lysogeny entails more economical and technological risks in probiotic Lactobacillus casei ⁄ paracasei bacteria than in other lactic acid bacteria, due to economic value. Lysogeny is widely spread among L. casei ⁄ paracasei strains. Siphophages CL1 and CL2, isolated from noninfected lysed-cultures of commercial L. paracasei A, are thermoresistant, have identical host spectrum, latent and burst times, whereas burst sizes are 148 and 85, respectively. Mitomycin C induction of L. paracasei A yielded prophage iA2, whose presence was confirmed on the strain genome and whose restriction patterns differed from phages CL1 and CL2. The latter shared several restriction fragments, probably indicating a common origin.