Study of anti-tumorigenic actions of Omega-3 fatty acid in a murine mammary gland adenocarcinoma by micro-XRF analysis

COMETTO VINCENTE, F.; PÉREZ C.A.; SORIA, E.A.; LEANI J.; FALCHINI, G.E.; PACUALINI, M. ; PÉREZ R.D.

Campinas

Workshop; 28th Annual Users Meeting LNLS/CNPEM; 2018

CNPEM

A total of 50 samples of dried neoplastic tissues fixed in paraffin blocks were evaluated by micro-XRF. The neoplastic tissues were previously implanted in 50 male and female BALB/c mice randomly distributed among two dietary groups: a normal diet (control) and a diet rich in ChO. The micro-XRF was calibrated to quantify traces of Ca, Fe, Cu and Zn on paraffin blocks. Standard materials with known amount of Ca, Fe, Cu and Zn were specifically developed for the quantification process. The standard materials were homogeneous solid solutions with uniform metal distributionsat micro-level scale and similar attenuation properties than dried soft biological tissues fixed in paraffin blocks.Thin slices of 30 microns of neoplastic tissues were analyzed by micro-XRF using synchrotron white beam focused at 20 microns by a KB optic. Scanned rectangular areas with edges of few millimeters were carefully selected with help of the histological image of the contiguous tissue. The results showed a significant decrease of metal concentrations in the neoplastic tissues for mice fed with ChO diet. A previous research showed that ChO inhibits growth and metastasis in a murine mammary gland adenocarcinoma model. These results are correlated with the observed decrease of metal concentrations which gives us the possibility to develop an improvedmodel of the anti-tumorigenic actions of Omega-3 fatty acid present in ChO diet .