INTERPRETATION OF MOLECULAR DYNAMICS ON DIFFERENT TIMESCALES IN UNILAMELLAR VESICLES USING FIELD-CYCLING NMR RELAXOMETRY

CARLA J. MELEDANDRI; JOSEFINA PERLO; EZEQUIEL FARRHER; DERMOT F. BROUGHAM; ESTEBAN ANOARDO

Torino, Italia

Conferencia; 6th Conference on field cycling NMR relaxometry; 2009

Both laboratory-frame and rotating-frame nuclear magnetic resonance relaxometry wereused to study the molecular dynamics in unilamellar liposome systems of diameterabout 100 nm composed of 1,2-dimyristoyl-sn-glycero-3-posphocholine (DMPC) or1,2-dioleoyl-sn-glycero-3-posphocholine (DOPC). The spin-lattice relaxationdispersions were interpreted in terms of clearly defined relaxation mechanismsassociated with the underlying molecular dynamics. The physical parameters obtainedfrom the analysis are consistent with values available in the literature obtained from arange of experimental techniques. We conclude that the methodology here employedcan therefore be validly applied to the study of liposomes of more complex formulation,to investigate the effect of composition on the important physicochemical properties ofthese model carrier systems.