IFEG   20353
INSTITUTO DE FISICA ENRIQUE GAVIOLA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Interaction of Riboflavin and Flavin-Mononucleotide with Derivatized Surfaces of Ag(111)
Autor/es:
L.B. AVALLE, M.L. VALLE, H. JONES, E. SANTOS
Lugar:
Sevilla, Espana
Reunión:
Congreso; 59th International Society of Electrochemistry; 2008
Resumen:
The riboflavin (RF) and flavin mononucleotide (FMN) are molecules involved in the cell respiratory chain. They take part in many processes such as energy metabolism, and are required for the metabolism of fats, carbohydrates, and proteins. RF is the component of the cofactors FAD and FMN, therefore required by all flavoproteins. As such, vitamin B2 is required for a wide variety of cellular processes, some of them involving the interaction with cell membranes. The interactions of these molecules with an adlayer of L-cysteine is investigated. Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) measurements at different applied potentials as well as Second Harmonic (SH) studies were performed in presence and in absence of the studied molecules (supporting electrolytes: 10mM HClO4 - pH=1, 10mM KClO4 - pH=5 and 10mM KOH – pH=12). The SH signal as a function of time for Ag(111) demonstrated that after a steady state signal has been reached, the electronic properties at the interface are mainly determined by the L-cysteine adlayer. The L-cysteine injection to the electrochemical cell removes the previously adsorbed RF molecule, as can also be confirmed by CV measurements