IFEG   20353
INSTITUTO DE FISICA ENRIQUE GAVIOLA
Unidad Ejecutora - UE
artículos
Título:
Association between As and Cu renal cortex accumulation and physiological and histological alterations after chronic arsenic intake
Autor/es:
RUBATO BIRRI, P.N.; PEREZ, R.D.; CREMONEZZI, D.; PEREZ, C.A.; RUBIO, M.; BONGIOVANNI, G.
Revista:
ENVIRONMENTAL RESEARCH
Editorial:
Academic Press
Referencias:
Lugar: London (UK); Año: 2010 vol. 110 p. 417 - 423
ISSN:
0013-9351
Resumen:
Arsenic (As) is one of the most abundant hazards in the environment and it is a human carcinogen. Related you excretory function, the kidneys in humans, animals models or naturally exposed fauna, are target organs for As accumulation and deleterious effects. Previous studies carried out by X-ray fluorescence spectrometry using synchrotron radiation (SR-mXRF) showed a high concentration of As in renal cortex from chronically exposed rats suggesting that this is a suitable model for studies on renal As accumulation. This accumulation was accompanied by an important increase in the copper (Cu) concentration. The present study focused on the localization of these elements along renal cortex and their correlation with physiological and histological As-related renal effects. Experiments were done on nine male Wistar rats, divided into tree experimental groups. Two groups received 100 mml sodium arsenite by drinking water during 60 and 120 consecutive days, respectively. Control group received water without sodium arsenite (< 50 ppb As). For histological analysis, 5 mm thick sections from kidneys were stained with hematoxylin and eosin. Biochemical analyses were used to determine concentrations of plasmatic urea and creatinine. The As and Cu mapping were carried out by SR-mXRF using a collimated white synchrotron spectrum (300 mm x 300 mm) on kidney slices (2 mm thick) showing an As and Cu co-distribution in renal cortex. Then, renal cortical slices (100 mm thick) were scanned with a focused white synchrotron spectrum (30 mm x 30 mm). Peri-glomerular accumulation of As and Cu at 60 and 120 days was found. The effects of 60 days arsenic consumption may be seen by a decreased Bowman´s space as well as a decreased plasmatic blood urea nitrogen (BUN)/creatinine ratio. However, major deleterious effects were seen on tubules at 120 days of exposition. This study supports the hypothesis that tubular accumulation of As-Cu may to have some bearing on the arsenic-associated nephrotoxicologial process.