INVESTIGADORES
CASATI Paula
artículos
Título:
Maize lines expressing RNAi to chromatin remodeling factors are similarly hypersensitive to UV-B radiation but exhibit distinct transcriptome responses.
Autor/es:
PAULA CASATI; VIRGINIA WALBOT
Revista:
Epigenetics
Editorial:
Landes
Referencias:
Lugar: Landes; Año: 2008 vol. 3 p. 216 - 229
ISSN:
1559-2308
Resumen:
RNAi knockdown lines targeting two putative chromatin factors (a methyl-CpG-binding domain protein MBD101 and a chromatin remodeling complex protein CHC101) exhibit identical phenotypic consequences after UV-B exposure including necrosis in adult leaves and seedling death.  Here we report that these RNAi lines exhibit substantially different transcriptome changes assessed on a 44K Agilent oligonucleotide array platform compared to each other and to UV-B tolerant non-transgenic siblings both before and after 8 h of UV-B exposure.  Adult maize leaves express ~26,000 transcript types under greenhouse growth conditions; after 8 h of UV-B exposure 267 transcripts exhibit an expression change in the B73 control line.  Most of these transcript abundance changes in B73 after UV-B treatment are not found in the two RNAi knockdown lines:  119 up-regulated transcript types and 128 down-regulated types are uniquely modulated in B73.  The mbd101 RNAi line shows many more line-specific transcript changes (897 up, 68 down) than either B73 or the chc101 line (72 up, 103 down).  By functional analysis, the largest category of genes with predicted functions affected by UV-B is the DNA/chromatin binding group.  Differential activation of suites of transcription factors in the control and transgenic lines are the likely explanation for the divergent transcriptome profiles.