PERINATAL ASPHYXIA INDUCED SHORT AND LONG TERM ALTERATIONS IN THE RAT SYNAPTIC ORGANIZATION AND FUNCTION.

KOLLIKER FRERS, R, QUARRACINO , HERRERA INÉS, ISOLABELLA MARCOS, ROUVIER S, LOGICA TORNATORE T, HOLUBIEC M, BARRETO G, GONZALEZ J, CASTILLA R, CAPANI F.

Rio Janeiro

Congreso; International Brain Research Congress; 2015

IBRO

Perinatal asphyxia (PA) induced short and long term synaptic and cytoskeletal alterations that has been associated with neuronal cell death following hypoxia. The lack of knowledge about the mechanisms underlying this dysfunction prompted us to investigate the changes in the synapse and neuronal cytoskeleton and related structures. For this study we used a well established murine model of PA. Full-term pregnant rats were rapidly decapitated and the uterus horns were placed in a water bath at 37 °C for different time of asphyxia. When their physiological conditions improved, they were given to surrogate mothers. One month, four month, 6 month and 18 month after PA rats were included in this study. Modifications were analyzed using photooxidation with phalloidin-eosin, conventional electron microscopy (EM), inmunocytochemistry and ethanolic phosphotungstic acid (E-PTA) staining combining with electron tomography and 3-D reconstruction techniques and molecular biology studies. After one month of the PA insult, an increase in the F-actin staining in neostriatum and hippocampus synapses was observed using correlative fluorescent electron microscopy for phalloidin-eosin. Mushroom-shaped spines showed the most consistent staining. Strong alterations in the dendrite and astroglial cytoskeleton were found at four months of PA. After six months of PA, postsynaptic densities (PSDs) of the rat neostriatum are highly modified. We observed an increment of PSDs thickness related with the duration and severity of the hypoxic insult. In addition, PSDs showed and increase in the ubiquitination level. Using 3-d reconstruction and electron tomography we observed showed clear signs of damage in the asphyctic PSDs. These changes are correlated with intense staining for ubiquitin. Finally, in 18 months old rat was observed a reduction in the number of synapses in the PA animals related with a decrease in BDNF staining. Using protocols of estradiol treatment we were able to revert some of these alterations using PI3K/Akt/GSK3. Overall these results demonstrate that synaptic dysfunction following PA might be produced by early changes in the actin organization and long-term misfolding and aggregation of proteins in the PSDs. Therefore, we hypothesize that the synaptic and neuronal cytoskeleton changes induced by PA in the rat CNS could lead to the cellular dysfunction and death in adult animals. Estradiol appears as a new therapeutic tool to slacken the damage induces by perinatal asphyxia on the CNS. Supported by UBACYT and CONICET.