INVESTIGADORES
CAPANI Francisco
congresos y reuniones científicas
Título:
CHARACTERIZATION OF THE EXPRESSION PATTERN OF THE THIOREDOXINS
Autor/es:
JUAN I. ROMERO(A), PABLO GALEANO(A), MARIA E. HANSCHMANN(B), CHRISTOPHER H. LILLIG(B), FRANCISCO CAPANI
Reunión:
Congreso; Segundo Encuentro de la Sociedad Argentina de Microscopia; 2012
Resumen:
Brain damage resulting from a combination of hypoxia and reduced cerebral blood flow
(ischemia) in the fetus and newborn infant remains a major cause of death and disability in children,
being cerebral palsy, mental retardation, and epilepsy among the most common complications
[1][2][3][4]. The incidence of severe perinatal asphyxia is estimated of about 1/1000 live births in
developed countries, having an incidence of 510/1000 live births in developing countries [5].
The production of reactive oxygen species (ROS) has been proposed as an important cause of
neuronal death and consequently brain damage after ischemiareperfusion [6]. Currently, several
therapeutic approaches for the destruction of oxygen free radicals generated during and following
hypoxiaischemia have been proposed. Such as the administration of specific enzymes known to
degrade highly reactive radicals to a non-reactive component, free radical inhibitors, and scavengers
[5].
At present, no individual neuroprotective agents have been proven safe and effective for the
protection of neonates from neurological sequels after a hypoxic-ischemic insult. The insight into
the biochemical and cellular mechanisms of neuronal injury with perinatal hypoxia ischemia helps
to provide interventions to interrupt those deleterious cascades, principally focusing on the potential
effects of free radical scavengers [7].
Nowadays, we look at Thioredoxins (Trxs) a crucial protein family for antioxidative defense, the
modulation of intra- and extracellular signalling pathways, the regulation of transcription factors,
and the modulation of immune response, and new functions of Trxs are described almost on a
weekly basis [8].
In the present work, we study the differential expression of the oxidoreductases of the Trxs of
proteins, and their role in the cellular response to oxidative stress in a modified model of perinatal
hypoxic-ischemia developed by Rice (1981) [1] and Levine (1960) [9]. At a molecular level we
have observed several remarkable differences in both abundance and regional distribution of Trxs
(this family) of proteins at different times. Combining Western Blot and light microscopy analysis,
we have observed some remarkable differential expression of these proteins in the most vulnerable
areas of the brain to hypoxia-ischemia.
REFERENCES
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Keywords: Perinatal hypoxia-ischemia, tioredoxins, CNS