INVESTIGADORES
CAPANI Francisco
congresos y reuniones científicas
Título:
Hypoxia induces actin cytoskeletal changes in neostriatal dendritic spines
Autor/es:
E. SARACENO1, L. AON2, V. BOTI1, M. KRUSE3, J. C. FERNANDEZ1, D. VALVERDE4, F. GATO1, D. MADUREIRA4, L. D. GIRALDEZ ALVAREZ4, H. CORINI1,3, *F. CAPANI1;; CAPANI F
Lugar:
San Diego CA USA
Reunión:
Congreso; Society for Neuroscience; 2007
Institución organizadora:
SFN
Resumen:
Program#/Poster#: 898.2/U26 Title: Hypoxia induces actin cytoskeletal changes in neostriatal dendritic spines Location: San Diego Convention Center: Halls B-H Presentation Start/End Time: Wednesday, Nov 07, 2007, 2:00 PM - 3:00 PM Authors: E. SARACENO1, L. AON2, V. BOTI1, M. KRUSE3, J. C. FERNANDEZ1, D. VALVERDE4, F. GATO1, D. MADUREIRA4, L. D. GIRALDEZ ALVAREZ4, H. CORINI1,3, *F. CAPANI1; 1Human Biochem, Sch. Med. Univ. Buenos Aires, 5 F Buenos Aires, Argentina; 2Human Biochem., Sch. of Medicine, Univ. of Buenos Aires, Buenos Aires, Argentina; 3IBYME-CONICET, Buenos Aires, Argentina; 4Univ. Federal da Bahía, Salvador, Brazil Cerebral hypoxia- ischemia damages synaptic proteins, resulting in protein aggregation and neuronal death. In previous work we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia. Since dendritic spines are highly concentrated in F-actin, changes in the F-actin organization could be related with the neuronal changes induced by hypoxia. In this work we studied the effects of hypoxia-ischemia on the actin cytoskeleton of neostriatal post-synaptic densities (PSD) in 60 days olds rats by combining photooxidation, 3D reconstruction and Western blot. In addition we studied the effects of reduced temperature from 37ºC to 15ºC on dendritic spines. Staining with phalloidin-alexa 488 followed by confocal microscopy analysis showed an increase of F-actin fluorescent staining in the neostriatum of hypoxic animals (Relative fluorescen intensity: control 15.1 ± 3 vs hypoxic animals 33.5 ±6 p < 001) . Electron microscopy and 3-D reconstruction confirmed these observations showing an increment in the number of F-actin staining spines in neostriatal excitatory synapses subjected to hypoxia (control 2 ± 0.1 vs hypoxic animals 3,6 ± 0.2 p < 001). Consistent with the microscopy data, Western blot revealed b-actin increase in hypoxic animals. The optic relative density measurement showed a significant difference between the control (1.43 ± 0,05) versus the hypoxic animals (2.27 ± 0.41). When hypoxia was induced under hypothermic conditions the changes observed were prevented. These results suggest that the cellular and subcellular alterations in neostriatal PSDs could be related to F-actin increase probably due to an overexpression of the b-actin gene (ACTB) after a hypoxic episode Disclosures:  E. Saraceno, None; L. Aon, None; V. Boti, None; M. Kruse, None; J.C. Fernandez, None; D. Valverde, None; F. Gato, None; D. Madureira, None; L.D. Giraldez Alvarez, None; H. Corini, None; F. Capani , None. Support: ANPCyT 15001 PID 5782 M020 [Authors]. [Abstract Title]. Program No. XXX.XX. 2007 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2007. Online.2007 Copyright by the Society for Neuroscience all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.   OASIS - Online Abstract Submission and Invitation System™ ©1996-2008, Coe-Truman Technologies, Inc.