Thioredoxins family proteins immunolocalization in the rat central nervous system.

AON-BERTOLINO, MARÍA LAURA; ROMERO, JUAN; SARACENO, GUSTAVO EZEQUIEL; GALEANO, PABLO; HOLUBIEC, MARIANA; BADORREY, SOL; MILEI, JOSÉ; LILLIG, CHRISTOPHER HORST; CAPANI, FRANCISCO.

Cordoba

Congreso; I Reunión Conjunta de Neurociencias. Taller Argentino de Neurociencias y Sociedad Argentina de Investigación en Neurociencias.; 2009

Glutamate excytotoxicity, oxidative stress and mitochondrial dysfunction are some of the process associated to neuronal damage and glial reaction triggered by a hypoxic ischemic injury. Although the most important function of the thiol redox proteins is the maintenance of the intracellular redox state, acting as antioxidants and reducing agents in redox signaling with oxidizing reactive oxygen species (ROS), no systematic data on the localization of these proteins in the Central Nervous System (CNS) is available until now. The aim of this work is to study the distribution of the following thioredoxins family proteins: Trx-1, Trx-2, TrxR-1, TrxR-2, Txnip, Grx-1, Grx-2, Grx-3, Grx-5, gGCS, Prx-1, Prx-2, Prx-3, Prx-4, Prx-5 and Prx-6, in Neostriatum, Hippocampus, Cerebellum, Spinal Cord, Substantia Nigra, Cerebral Cortex and Retina, which are the most vulnerable areas to hypoxic ischemic injury. While previous studies had suggested that these proteins are distributed in most of the cell types and regions of the CNS, we have observed several differences in intensity and regional distribution in these areas. The present study is the first effort to precise the localization of these proteins and could contribute to reveal new insights about the role of the oxidative pathway in the pathogenesis of the hypoxic-ischemic brain injury and neurodegenerative associated diseases. Supported by UBACYT M407, PIP 5784, PICT 15001.