M6a glycoprotein is involved in neuronal plasticity after perinatal asphyxia. Huerta Grande, Córdoba, Argentina:

SARACENO, GUSTAVO EZEQUIEL; BROCCO, MARCELA ADRIANA; GALEANO, PABLO; ROMERO, JUAN; HOLUBIEC, MARIANA; CAPANI, FRANCISCO

I Reunión Conjunta de Neurociencias. Taller Argentino de Neurociencias y Sociedad Argentina de Investigación en Neurociencias

Congreso; I Reunión Conjunta de Neurociencias. Taller Argentino de Neurociencias y Sociedad Argentina de Investigación en Neurociencias; 2009

SAN

During perinatal asphyctic episode produces a synaptic dysfunction that has been related to neuronal death, although this mechanism is not fully understood yet.  Previously, we showed in 30 days old rats, that asphyxia increased actin cytoskeleton in hippocampal post-synaptic densities. In additiion, using electron and confocal microscopy, we observed an increase in dendritic spine number in Stratum radiatum of CA1 area from asphyxiated animals. Moreover, newly formed filopodia were observed in those animals probably as a consequence of neuronal plasticity induced by asphyxia.  In addition we also demonstrated that the glycoprotein M6a is involved in hippocampal neuronal plasticity by promoting filopodium/spine formation and is regulated by stress. Taken together all of these previous observations, the main aim of this work is to investigate the expression of M6a in 30 days old rats that were subjected to perinatal asphyxia (PA) by real time RT-PCR and immunocytochemistry. Real time PCR assays revealed an over expression of M6a mRNA in hippocampal formation from asphyxiated animals in comparison to control animals. Immunocytochemistry also showed an increase in M6a staining in hippocampus CA1 area from treated animals. These results suggest that M6a glycoprotein could be involved in the dynamic of dendritic spines changes in asphyctic animals. Therefore, these observations indicate that perinatal asphyxia could affect neuronal plasticity mediated for the changes in the expression of M6a glycoprotein. UBACYT M407; PIP 5784.