Influence of Bile on Lactobacillus reuteri Subjected to Freeze-drying

FONT DE VALDEZ, GRACIELA; MARTOS, GLADYS; TARANTO, MARIA PIA; LORCA, GRACIELA; OLIVER, GUILLERMO; RUIZ HOLGADO, AIDA

JOURNAL OF DAIRY SCIENCE

AMER DAIRY SCIENCE ASSOC-ADSA

Lugar: USA; Año: 1997 vol. 80 p. 1655 - 1658

0022-0302

The effect of bile on b-galactosidase activity and cell viability was investigated using two strains of Lactobacillus reuteri that were subjected to freezedrying. In the presence of 0.15% oxgall, bgalactosidase activity of the whole cells was significantly increased. After lyophilization, the cultures that had been treated with oxgall showed a low survival rate without changes in b-galactosidase activity. The poor resistance of the cells to damage from freeze-drying might be related to the presence of membranous structures containing simple folds and buds of the cell membrane, as was  observed by transmission electron microscopy.