INVESTIGADORES
MIRANDA Maria Victoria
artículos
Título:
Simple production of hydrophobin-fused domain III of dengue envelope protein and induction of neutralizing antibodies against the homotypic serotype of dengue virus
Autor/es:
CEREZO, JULIETA; ALEXANDRA TARGOVNIK; EMILIA SMITH; GONZALEZ MAGLIO ; LUPPO; MORALES ALEJANDRA; MARIA VICTORIA MIRANDA; JULIAN RODRIGUEZ TALOU
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2020 vol. 42 p. 419 - 428
ISSN:
0141-5492
Resumen:
Hydrophobin-fused domain III of dengueenvelope proteins serotypes 1 and 2 were expressed inRachiplusia nu larvae and purified by aqueous twophasesystem. This biotechnological approach ofhydrophobin-fused proteins, which allowed obtaining97.7 lg/larva of fusion protein DomIII serotype 1 and61.4 lg/larva of fusion protein DomIII serotype 2,represents an integrated strategy for simple productionof recombinant antigens. Purified fusion proteinsinduced serotype-specific neutralizing antibodieswithout cross-reaction against other serotypes andarboviruses after mouse immunization. hydrophobinfuseddomain III of dengue envelope protein could bea promising strategy for easy and low-cost production of components of a tetravalent sub-unit vaccineagainst dengue.
