Immobilisation of soybean seed coat peroxidase on its natural support

MAGRI, MARÍA LAURA; M.N. LOUSTAU; MARÍA VICTORIA MIRANDA; OSVALDO CASCONE

BIOCATALYSIS AND BIOTRANSFORMATION

TAYLOR & FRANCIS LTD

Año: 2007 vol. 25 p. 98 - 102

1024-2422

Soybean seed coat peroxidase (SBP; EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any SBP,whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found to be 1%. Immobilisation shifted the optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat retained its activity over a 4-week period, and reusability assays showed that treated seed coats could be reused once for phenol removal. Polyethylene glycol (PEG)increased the stability of phenol degradation activity. In addition, the phenolic polymer was adsorbed on to seed coats, thus making removal of the polymeric product easier.