INVESTIGADORES
CHEHIN Rosana Nieves
artículos
Título:
Fourier-transformed infrared assay for the determination of positional specificity of non-specific and 1,3-specific lipases
Autor/es:
FARÍAS R; CHEHÍN R; CANELA R
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
Springer Publishers
Referencias:
Año: 2000 vol. 22 p. 905 - 908
ISSN:
0141-5492
Resumen:
Lipases can be classified in terms of specificity toward the position of the acyl group of triacylglycerols into two groups: 1,3-specific and non-specific. The positional specificity of the lipases is usually determined by TLC (Commenil et al. 1995, Li-Zu-Yi & Ward 1993, Mozaffar & Weete 1993), HPLC (Ergan & Andre 1989, Veeraragavan 1990, Uzawa et al. 1993) or GC (Bereuter & Lorbeer 1995) analysis of the reaction products obtained after various reaction times with triacylglycerols as substrate. In the present paper we report a new, simple and fast infrared spectroscopy method for the characterization of both 1,3-specific and non-specific lipases using a thioester analogue of triacylglycerol: 2,3-dimercapto-1-propanol tributyrate. The method is based on the analysis of specific infrared bands that show the ester and thioester groups of the thioester analogue. It represents an alternative to another spectrophotometric method already described for our group with the same purpose (Farias et al. 1997).et al. 1995, Li-Zu-Yi & Ward 1993, Mozaffar & Weete 1993), HPLC (Ergan & Andre 1989, Veeraragavan 1990, Uzawa et al. 1993) or GC (Bereuter & Lorbeer 1995) analysis of the reaction products obtained after various reaction times with triacylglycerols as substrate. In the present paper we report a new, simple and fast infrared spectroscopy method for the characterization of both 1,3-specific and non-specific lipases using a thioester analogue of triacylglycerol: 2,3-dimercapto-1-propanol tributyrate. The method is based on the analysis of specific infrared bands that show the ester and thioester groups of the thioester analogue. It represents an alternative to another spectrophotometric method already described for our group with the same purpose (Farias et al. 1997).