INVESTIGADORES
SALA Adriana Andrea
congresos y reuniones científicas
Título:
Q1a3a NATIVE-AMERICAN Y-HAPLOGROUP DETECTION IN DNA QUANTIFICATION STEP
Autor/es:
GINART SANTIAGO; CAPUTO, MARIELA; CORACH DANIEL; SALA ANDREA
Lugar:
Praga
Reunión:
Congreso; 28th Congress of the International Society for Forensic Genetics-ISFG-2019.; 2019
Institución organizadora:
International Society for Forensic Genetics-ISFG
Resumen:
Theknowledge of ancestral genetic information of a male contributor to a forensic samplecan provide investigative clues such as assessing population of origin of thesample donor. Currently, this information is available after SNPs analysis. Somehuman DNA quantification kits might simultaneously provide information aboutgender or DNA degradation extent, but none supply information concerning geneticancestry. Here we present a system of quantification of human DNA that detects Q1a3anative-American haplogroup (hg) by typing M3-Q3 C/T SNP. Detection is made by RealTime PCR followed by melting temperature (Tm) of one specific Y-chromosomefragment (58bp long) that determines Q1a3a hg (TmT=76 °C; TmC=77°C=).We analyzed herein the usefulness of this diagnostic tool prior to STRs typing step.Fifty reference male DNA samples (blood, saliva and muscle from autopsy) wereanalyzed. DNA concentration ranged from 0,01 to 28 ng/uL. Thirteen werecharacterized as native-American (TmT = 76,02±0,04) while thirty-seven as non-native-American (TmC=77,13 ±0,06). Theseresults were confirmed with Y-STRs haplotypes and White Athey´s hg predictor. Fifty casework samples containing male DNA were successfully haplogrouped andthe STRs profiles, subsequently obtained, correlated well with quantitationresults. The reaction cocktail also includes short (152 bp; Tm=84.9°C) and long(244 bp; Tm=83.1°C) fragments, allowing to infer DNA integrity. This system isa quick tool for choosing the most suitable Y-chromosomedatabase for statistical evaluations and, simultaneously, allows quality evaluationof degraded samples. Since the detection chemistry is based on an intercalatingfluorochrome, it is cost-effective and compatible with any real-time PCRplatform that allows melting analysis.
