INVESTIGADORES
ALONSO Daniel Fernando
artículos
Título:
Effects of the synthetic vasopressin analog desmopressin in a mouse model of colon cancer
Autor/es:
GV RIPOLL; J GARONA; G HERMO; DE GOMEZ; DF ALONSO
Revista:
ANTICANCER RESEARCH
Editorial:
INT INST ANTICANCER RESEARCH
Referencias:
Año: 2010 vol. 30 p. 5049 - 5054
ISSN:
0250-7005
Resumen:
Experimental and clinical data indicated that perioperative
administration of the hemostatic peptide desmopressin (DDAVP) can
inhibit progression of residual metastatic cells. The compound seems to
act by inducing an agonist effect on specific V2 vasopressin membrane
receptors present in both tumor cells and endothelial cells. Here we
explored the antitumor effects of DDAVP in cultured colon carcinoma
cells and in a syngeneic Balb/c mouse model. Both human Colo-205 and
mouse CT-26 colon carcinoma cell lines expressed the V2 receptor, as
revealed by immunofluorescence. DDAVP (at doses ranging from 100 ng/ml
to 1 μg/ml) exerted a modest but significant antiproliferative effect on
cultured CT-26 and Colo-205 cells. In vivo, DDAVP (2 intravenous doses
of 2 μg/kg) reduced accumulation of ascites and formation of intestinal
tumor nodules in mice intraperitoneally inoculated with CT-26 cells.
Perioperative administration of DDAVP significantly inhibited tumor
progression in animals surgically implanted in the spleen with CT-26
cells, and caused some reduction in liver metastasis. Although DDAVP and
5-fluorouracil demonstrated additive cytostatic effects in vitro, no
antitumor effects were observed in this study in mice receiving a single
cycle of chemotherapy (25 mg/kg) in combination with the peptide. Our
data suggest that DDAVP may be potentially used to minimize spread or
survival of residual malignant cells during surgical procedures for
colon and other gastrointestinal tumors.