INVESTIGADORES
ALONSO Daniel Fernando
artículos
Título:
Detection and characterization of N-glycolylated gangliosides in Wilms tumor by immunohistochemistry
Autor/es:
AM SCURSONI; L GALLUZZO; S CAMARERO; N POZZO; MR GABRI; C MATEO; AM VAZQUEZ; DF ALONSO; MT DAVILA
Revista:
PEDIATRIC AND DEVELOPMENTAL PATHOLOGY
Editorial:
ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS
Referencias:
Año: 2010 vol. 13 p. 18 - 23
ISSN:
1093-5266
Resumen:
Gangliosides are glycolipids present on the cell surface. The
N-glycolylated ganglioside NeuGc-GM3 has been described in some
neoplasms, such as breast carcinoma and melanoma, but is usually not
detected in normal human cells. Our aim was to evaluate the presence of
NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy
tumors were grouped into different histologic subtypes considering the
main preserved component. Formalin-fixed, paraffin-embedded tumor
samples were cut into 5-microm sections. The monoclonal antibody 14F7, a
mouse IgG1 that specifically recognizes NeuGc-GM3, and a
peroxidase-labeled polymer conjugated to secondary antibodies were used.
Sections from breast carcinoma were employed as positive controls.
Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining
was stronger in the epithelial component, with a membrane pattern and
cytoplasmic diffusion. The stromal component expressed cytoplasmic
NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of
adjacent renal tissue were also positive, but no expression of NeuGc-GM3
was detected in nontumoral fetal kidney. Until now, the expression of
N-glycolylated gangliosides in pediatric solid tumors has not been
investigated. The present study evidenced the expression of NeuGc-GM3 in
a high proportion of Wilms tumors, suggesting its potential utility as a
specific target of immunotherapy